Fig. 7

N/OFQ reversibly hyperpolarizes and decreases firing in POMC neurons from eGFP-POMC mice via an NOP receptor-mediated mechanism. Membrane current traces show the reversible hyperpolarization and decrease in firing cause by N/OFQ (a; n = 13) and the abolition of these effects in the presence of BAN (b; n = 11). The composite bar graphs in (c, d) show the abrogation of N/OFQ-induced hyperpolarization and decrease in firing due to BAN application. Bars are representative of the means, while lines represent 1 SEM of the membrane hyperpolarization (c) and the normalized change in firing rate (d) caused by N/OFQ alone and in combination with BAN. *p < 0.05, Student’s t test (c) or Kruskal-Wallis/ median-notched box-and-whisker plot (d)