Fig. 14
Intra-ARC N/OFQ alters energy intake and expenditure in a diet-dependent fashion. N/OFQ (0.3 nmol) significantly increased cumulative energy intake (a) in chow- and HFD-fed wildtype male mice compared to saline-treated controls. It also increased meal frequency (b), meal size (c), and rate of consumption (d) in a diet-dependent fashion. In addition, it decreased indices of energy expenditure including O2 consumption (e) and CO2 production (f) and altered metabolic heat production (g). Bars represent means and lines 1 S.E.M. of the cumulative food intake, meal frequency, meal size, rate of consumption, O2 consumption, CO2 production, and metabolic heat production, seen in male mice injected with either N/OFQ (0.3 nmol; n = 6), or its filtered 0.9% saline vehicle (0.2 μL; n = 6) *p < 0.05 relative to saline vehicle; repeated-measures, multi-factorial ANOVA/LSD; #p < 0.05 relative to chow-fed controls; repeated measures, multi-factorial ANOVA/LSD