Fig. 5

a Upper panel: Representative immunoblots showing PHB and OPA1 expression levels in visceral adipose tissue (VAT) from gonadectomized and sham-operated Mito-Ob mice and wild-type mice. Middle and lower panel: Histograms showing quantification of PHB and OPA1 levels. Data are presented as mean ± SEM (n = 4). * p < 0.05 represent significant differences between orchiectomized or ovariectomized Mito-Ob mice and sham control mice. b. Histograms showing mitochondrial DNA (mtDNA) copy number in VAT form gonadectomized Mito-Ob and wild-type. Data are presented as mean ± SEM (n = 4). * p < 0.05 represent significant differences between ovariectomized Mito-Ob mice and sham control mice. No significant difference was found between sham-operated and gonadectomized wild-type mice. Orc—orchiectomized; Ovx—ovariectomized