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Table 3 Antibody specifications

From: Sex and strain dependent differences in mucosal immunology and microbiota composition in mice

Specificity

Clone name

Fluorchrome

Concentration

Dilutiona

Supplier

CD3

17A2

Pacific blue

0.5 mg/ml

80×

Biolegend

CD8

53–6.7

A700

0.5 mg/ml

50×

Biolegend

CD4

GK1.5

PE-Cy7

0.2 mg/ml

100×

Biolegend

CD69

H1.2F3

FitC

0.5 mg/ml

25×

Biolegend

α4β7

DATK32

APC

0.2 mg/ml

25×

Biolegend

CD62L

MEL-14

Biotin

0.5 mg/ml

200×

Biolegend

  

Streptavidin-Pacific Orange

1 mg/ml

100×

ThermoFisher

CD44

IM7

APC-Cy7

0.2 mg/ml

100×

Biolegend

  1. aDilution used in a total volume of 25 μl supplemented with PBS + 10% FCS