Skip to main content

Table 3 Antibody specifications

From: Sex and strain dependent differences in mucosal immunology and microbiota composition in mice

Specificity Clone name Fluorchrome Concentration Dilutiona Supplier
CD3 17A2 Pacific blue 0.5 mg/ml 80× Biolegend
CD8 53–6.7 A700 0.5 mg/ml 50× Biolegend
CD4 GK1.5 PE-Cy7 0.2 mg/ml 100× Biolegend
CD69 H1.2F3 FitC 0.5 mg/ml 25× Biolegend
α4β7 DATK32 APC 0.2 mg/ml 25× Biolegend
CD62L MEL-14 Biotin 0.5 mg/ml 200× Biolegend
   Streptavidin-Pacific Orange 1 mg/ml 100× ThermoFisher
CD44 IM7 APC-Cy7 0.2 mg/ml 100× Biolegend
  1. aDilution used in a total volume of 25 μl supplemented with PBS + 10% FCS