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Fig. 5 | Biology of Sex Differences

Fig. 5

From: Sex and strain dependent differences in mucosal immunology and microbiota composition in mice

Fig. 5

Effect of sex and strain on maturation of T lymphocytes in the mesenteric lymph nodes. Gating strategy for determination of T cell subsets in the mesenteric lymph nodes (a). Lymphocytes were gated based on size and scatter in the forward side scatter plot. T cells were determined by selecting CD3+ cells. Within the CD3+ cells, CD8+ (Tc cells) and CD4+ (Th cells) cells were selected. Within both the CD8+ and CD4+ population, the percentage of CD62L and CD44 were measured. All isotype controls were set at 1%. Frequency of CD62L+CD44 naive CD8 (b) and CD4 (e), CD62L+CD44+ central memory CD8 (c) and CD4 (f) and CD62LCD44+ effector memory CD8 (d) and CD4 (g) in the mesenteric lymph nodes of male and female BALB/c and B6 mice (10 mice per group). T cytotoxic cells are expressed as the frequency of CD8+ cells within the CD3+ population, whereas T helper cells are expressed as the frequency of CD4+ cells within the CD3+ population. Results are shown as mean + SEM and were tested for overall strain and sex effects using a two-way ANOVA followed by a Bonferroni post hoc test to test for strain-specific sex effects when interaction was found. Significant strain effects are indicated with solid lines and significant sex effects are indicated with dashed lines (p < 0.05)

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