Fig. 3

Expression of the novel candidate genes in AmhCre Sox9floxflox XY gonads. a Immunofluorescence of the wild-type and Sox9 knockout gonad at E13.5. Sox9 protein is lost from the testicular cords (white arrows) in the Amh-Cre Sox9floxflox mice yet the testicular cords remain intact, as shown by the laminin stain. Sox9/Laminin is shown in green, and nuclei stained with DAPI are shown in blue. b Expression levels of candidate genes in AmhCre Sox9floxflox XY gonads (red) and WT B6 XY gonads (blue). Expression in WT B6 female gonads is also shown (XX in green). The expression values are shown in TMM values (trimmed mean of M values). RNA-seq was done n = 3 with six pooled E13.5 gonads in each sample. Error bars represent standard error of the mean. Asterisks indicate significantly differentially expressed genes based on an adjusted P value < 0.05