Sex differences in primary muscle afferent sensitization following ischemia and reperfusion injury

Table 1 Percent changes in mRNA from C7/C8/T1 DRGs in male and female mice

Gene	Naïve female expression	I/R-induced upregulation		Two-way comparisons
Gene	(Relative to naïve males)	Male	Female	Injury	Sex	Interaction
ASIC1	15.3 ± 17%	77.1 ± 13% *	34.8 ± 17%	< 0.001	0.955	0.193
ASIC3	− 9.7 ± 12%	85.0 ± 9% *	10.6 ± 11%	< 0.001	< 0.001	0.002
IL1r1	− 10.1 ± 17%	74.0 ± 10% *	95.0 ± 23% *	< 0.001	0.698	0.659
P2X3	− 59.7 ± 29% *	75.8 ± 23% *	181.9 ± 21%*	< 0.001	< 0.001	0.112
P2X5	− 33.7 ± 18%	50.2 ± 8% *	63.2 ± 22% *	< 0.001	0.004	0.740
TRPM8	− 46.4 ± 20% *	17.8 ± 19%	93.4 ± 24% *	0.005	0.01	0.085
TRPV1	− 40.1 ± 14% *	42.3 ± 23%	133.7 ± 17% *	< 0.001	0.078	0.098

Values indicate percentage change in mRNA expression of naïve females relative to naïve males and 1d I/R animals of both sexes relative to sex- and age-matched naïve controls ± error of the difference in means (n = 4–11 per condition). Final * P < .05 obtained via false discovery rate correction following two-way ANOVA on raw sample ∆Cts

ISSN: 2042-6410