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Fig. 5 | Biology of Sex Differences

Fig. 5

From: SP-A2 contributes to miRNA-mediated sex differences in response to oxidative stress: pro-inflammatory, anti-apoptotic, and anti-oxidant pathways are involved

Fig. 5

Effect of O3 in males. a mRNA levels of GAPDH, SOD2, CAT, IL-6, STAT3, BCL2, FOXO1, FOXO3, BECN1, IKKβ, and NF-kB-p65 genes were measured in AM of male SP-A2 mice 4 h after O3 exposure. mRNA levels were measured by qRT-PCR and normalized to GAPDH. STAT3 mRNA levels were significantly increased by 5-fold (p < 0.05), while the levels of other mRNAs did not change significantly compared to FA exposure. Inset depicts protein expression levels of STAT3, NF-kB-p65, FOXO1, CAT, and SOD2 in AM of male SP-A2 mice 4 h after O3 exposure. Protein levels were measured by Western blot analysis and normalized to GAPDH. b mRNA levels of the above mentioned genes were measured by qRT-PCR 18 h post exposure. In male SP-A2, O3 exposure significantly increased BCL2 mRNA levels compared to FA (p < 0.05). The mRNA levels of SOD2, CAT, IL-6, FOXO3, BECN1, and IKKβ were significantly decreased compared to FA (p < 0.05). c In KO, mRNA levels of SOD2 and CAT were significantly increased (p < 0.05) 18 h post exposure, while the levels of other mRNAs did not change significantly compared to FA. Data were generated using 3 animals/group, n = 18, and 3 replicate/animal

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