Fig. 4

The effect of gonadectomy and OxS on AM miRNA expression profiles of SP-A2 mice. The differentially expressed miRNAs in SP-A2 non-gonadectomized (NGx) and gonadectomized (Gx) mice were identified after normalizing to corresponding NGx and Gx KO. NGx shows the miRNAs (n = 37) that changed significantly (≥ 2-fold) in OxS when males were compared to females. Gx depicts the comparison of Gx values (male vs female) to NGx (male vs female). Out of the same 37 miRNAs (found to have their levels increased in NGx), 2 miRNAs (5.4%) showed a significant increase (≥ 2-fold) and 35 miRNAs (94.59%) showed a significant decrease (≤ 2-fold). Inset depicts the comparison, of the 37 differentially expressed miRNAs, between Gx male and female. Out of 37 miRNAs studied, 17 miRNAs (45.9%) are significantly increased in female (≥ 2-fold), and 7 miRNAs (18.9%) are significantly increased in male (≥ 2-fold)