Fig. 1
Volcano plots indicating the statistical significance of SP-A1 AM miRNome expression levels compared to SP-A KO under FA or O3 exposure for males and females. The x-axis plots the log2 of the fold changes, while the y-axis plots the −log10 of their p values based on t test of the replicate raw Ct data (see the “Methods” section). Each plot has 3 vertical lines. The middle vertical line that is graded corresponds to 0 changes. The lines on either side represent ≥ 2-fold differences. Dots in the volcano plots above the blue horizontal line identify fold changes with statistical significance of at least the Bonferroni corrected p < 0.0166. The red and green dots represent miRNAs that were upregulated ≥ 2-fold and downregulated ≤ − 2-fold, respectively, compared to KO. Black dots signify miRNAs that were regulated less than 2-fold times (i.e., − 2 ≤ x ≤ 2; x is the fold change). a Male SP-A1 mice compared to KO exposed to FA. b Female SP-A1 mice compared to KO exposed to FA. c Male SP-A1 mice compared to KO after O3 exposure. d Female SP-A1 mice compared to KO after O3 exposure. The shaded gray areas show the differences in the miRNAs that are highly and significantly regulated between the two sexes and the two conditions compared to KO. The shaded gray areas with a broken outline are to highlight the significantly changed miRNAs in SP-A1 male O3 exposed mice and SP-A2 male O3 exposed mice (Fig. 2). The x-axis on a is broken to allow comparison between all panels