Table 1 Summary of existing simplex PCR methods for determining genetic sex of mice

Uba1 (X)

Uba1y (Y)

217 bp (X)

198 bp (Y)

XX 217 bp

XY 198 bp; 217 bp

Forward primer is mismatched to Uba1y.

Small difference (19 bp) in amplicon size necessitates extended gel run time.

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Kdm5c (X)

Kdm5d (Y)

331 bp (X)

302 bp (Y)

XX 331 bp

XY 331 bp; 302 bp

Primers are mismatched to Kdm5c.

Small difference (29 bp) in amplicon size necessitates extended gel run time.

[13]

Xlr (X)

Sly (Y)

685 bp (X)

280 bp (Y)

XX 685 bp, ~ 660 bp, ~ 480 bp

XY 280 bp

Additional products amplified from X chromosome.

Failure to amplify X chromosome in XY samples.

[14]