Advertisement
Table 1 Summary of existing simplex PCR methods for determining genetic sex of mice
| Targets | Predicted products | Observed products | Potential limitation | Reference |
|---|---|---|---|---|
| Uba1 (X) Uba1y (Y) | 217 bp (X) 198 bp (Y) | XX 217 bp XY 198 bp; 217 bp | Forward primer is mismatched to Uba1y. Small difference (19 bp) in amplicon size necessitates extended gel run time. | [12] |
| Kdm5c (X) Kdm5d (Y) | 331 bp (X) 302 bp (Y) | XX 331 bp XY 331 bp; 302 bp | Primers are mismatched to Kdm5c. Small difference (29 bp) in amplicon size necessitates extended gel run time. | [13] |
| Xlr (X) Sly (Y) | 685 bp (X) 280 bp (Y) | XX 685 bp, ~ 660 bp, ~ 480 bp XY 280 bp | Additional products amplified from X chromosome. Failure to amplify X chromosome in XY samples. | [14] |
