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Table 1 Summary of existing simplex PCR methods for determining genetic sex of mice

From: Genetic sex determination of mice by simplex PCR

Targets Predicted products Observed products Potential limitation Reference
Uba1 (X) Uba1y (Y) 217 bp (X) 198 bp (Y) XX 217 bp XY 198 bp; 217 bp Forward primer is mismatched to Uba1y. Small difference (19 bp) in amplicon size necessitates extended gel run time. [12]
Kdm5c (X) Kdm5d (Y) 331 bp (X) 302 bp (Y) XX 331 bp XY 331 bp; 302 bp Primers are mismatched to Kdm5c. Small difference (29 bp) in amplicon size necessitates extended gel run time. [13]
Xlr (X) Sly (Y) 685 bp (X) 280 bp (Y) XX 685 bp, ~ 660 bp, ~ 480 bp XY 280 bp Additional products amplified from X chromosome. Failure to amplify X chromosome in XY samples. [14]
  1. The three existing methods of genetic sex determination of mice by simplex PCR are summarized, detailing expected and observed PCR products and the potential limitations of each method