Fig. 7

Effects of 17 α-E2 and 17 β-E2 on p65 and ERα protein expression in 3T3-L1 differentiated cells. a Nuclear and cytoplasmic fractions were extracted for Western blotting (WB) detection of p65. b Total protein was extracted for WB detection of p65. c Microscopy images of immunofluorescence labeling of p65 (red) in 3T3-L1 cells. Nuclei were stained with DAPI. d Total protein was extracted for WB detection of ERα. e Microscopy images of immunofluorescence labeling of ERα (green) in 3T3-L1 cells. Nuclei were stained with DAPI. Data are presented as mean ± SEM values. Asterisk indicates P < 0.05 compared to control group (C). Pound indicates P < 0.05 × LPS group. (n = 4 independent rounds of cells for WB, and n = 3 independent rounds of cells for immunofluorescence)