Fig. 4
From: The effects of 17 alpha-estradiol to inhibit inflammation in vitro
Effects of 17 α-E2 and 17 β-E2 on modulation of LPS-induced inflammatory markers in primary pre-adipocyte cells derived from C57BL/6J female and male mice. Cells were pre-treated with E2s for 1 h, followed by 2 h LPS treatment. Relative mRNA expression of Tnf-α (a), Il-6 (b), and Esr1 (c). Data are presented as mean ± SEM values. Asterisk indicates P < 0.05 compared to control group (C). Pound indicates P < 0.05 × LPS group. Dollar sign indicates P < 0.05 × LPS + 17 α-E2 group. Ampersand indicates P < 0.05 × females. (n = 3 independent rounds of cells)