Fig. 2
From: The effects of 17 alpha-estradiol to inhibit inflammation in vitro
Effects of 17 α-E2 and 17 β-E2 on mouse embryonic fibroblast (MEF) cells derived from C57BL/6J WT and ERKO male mice pre-treated overnight with 17 α-E2 (A, C, and E), or 17 β-E2 (B, D, and E) for 12 h, followed by 2 h LPS. Relative mRNA expression of Esr-1 (a–b), Tnf-α (c–d), and Il-6 (e–f). Data are presented as mean ± SEM values. Asterisk indicates P < 0.05 × control group (C). Pound indicates P < 0.05 × LPS group. Dollar sign indicates P < 0.05 × LPS + 17 α-E2 group. Two asterisks indicates P < 0.05 × LPS + 17 β-E2 group (n = 3 independent rounds of cells)