Fig. 4

Prdm14-associated enhancer response correlates with Prdm14 dosage. a Schematic of vectors used in luciferase assays. b XX and XY ES cells were each transfected with vectors containing either a Prdm14- or Trim24-responsive enhancer. Luciferase activity was higher in XX ES cells for the Prdm14-responsive enhancer. Trim-24 mRNA levels were equal in XX and XY ES cells and the Trim24-responsive enhancer showed no difference in enhancer activity. Error bars represent SEM. Samples were normalized to an empty vector control. c Top, schematic of the luciferase plasmid and the binding motif for Prdm14. Both the deletion and scrambling of the motif reduced the sex-specific differences and decreased overall luciferase activity in all biological replicates