Fig. 7From: HDAC9 is an epigenetic repressor of kidney angiotensinogen establishing a sex differenceAssociation of HDAC9 with AGT promoter. ChiP assays were performed to test interaction between HDAC9 and AGT promoter region. Two primer sets for a proximal region (from −437 to −951 bp) and a distal region (from −1,427 to −1,879 bp) of AGT promoter were designed and used in the assay (summarized in panel a). An anti-HDAC9 antibody (b) and anti-histone H3 and H4 antibodies (c) were used in the assays. Rabbit IgG was used as a negative controlBack to article page