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Fig. 3 | Biology of Sex Differences

Fig. 3

From: Sex differences in rat placental development: from pre-implantation to late gestation

Fig. 3

Sexually dimorphic outcomes of zonal volumes within the placenta from E13 to E20 during gestation. Volumes of placental zones. WP whole placenta, LAB labyrinth, JZ junctional zone, DEC decidua were estimated at E13 (a), E15 (c) and E20 (e). b Haematoxylin and eosin stain of an E13 placenta. d In situ hybridisation for Mest at E15 and f Isolectin B4 at E20, both marking fetal endothelial cells of the fetal blood space of the labyrinth. See Fig. 4 for higher magnification images. White (male) and black (female). Data shows mean ± SEM, analysed by two-way ANOVAs for sex with zone. *P < 0.05 post-hoc by Student’s t test. For E13 N = 6–7 samples/sex from 4 litters, E15–N = 8–9 samples/sex from 6 litters, E20 N = 11–12 samples/sex from 6 litters

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