Fig. 1
From: Sex differences in rat placental development: from pre-implantation to late gestation
Analysis of pre-implantation development at E5 for male and female in vivo-derived embryos. a Representative image of E5 blastocyst immunolocalised with CDX2 (green) marking the trophectoderm, and the counterstain for nuclei with DAPI (blue). b Total cell count. c Inner cell mass (ICM) count. d Trophectoderm (TE) count. e Ratio of trophectoderm to inner cell mass (TE:ICM). f Percentage of inner cell mass to total cell count (% ICM). Data shows mean ± SEM, analysed by Student’s t test. Pooled embryos from N = 22–27 male, N = 30–37 female from 11–13 litters