Fig. 4

Restoration of estrogen receptor beta expression in the lungs of ApoE-deficient middle-aged female mice with estrogen therapy. a Representative Western immunoblot images (upper) and bar graphs (lower) in the lungs from MA ApoE-deficient mice treated with PBS (control, n = 4), MCT (PH group, n = 3), and MCT with E2 (E2 group, n = 5) showing ERα and ERβ protein expression. *p < 0.05 vs. control, #p < 0.05 vs. PH (one-way ANOVA). Values are expressed as mean ± SEM. b Representative Western immunoblot images (upper) and bar graphs (lower) in RV from MA ApoE-deficient mice treated with PBS (control, n = 4), MCT (PH group, n = 3), and MCT with E2 (E2 group, n = 5) showing ERα, ERβ, and GPR30 expression. Values are expressed as mean ± SEM. c Western immunoblot images showing expression of GPR30 only in the RV, but not in the lungs. In all blots Vinculin was used as loading control and expression of each protein was normalized to Vinculin