Fig. 7

4-Vinylcyclohexene diepoxide induced ovarian failure reduced TH levels and CL-induced browning of gWAT of female mice. a Immunoblot analysis of UCP1 and TH protein in gWAT of vehicle- and VCD-treated mice after 3 days of CL treatment and control mice. Two-way ANOVA revealed significant main effects of VCD treatment in TH (p = 0.0037) and UCP1 expression (p = 0.015). Significant differences between controls and VCD-treated female mice were determined by post hoc pairwise comparison with Bonferroni correction (mean ± SEM; n = 4, *p < 0.05, **p < 0.01) (b). Basal and oligomycin- and rotenone-induced oxygen consumption rate of gWAT obtained from vehicle- and VCD-treated mice treated with CL for 3 days. c. qPCR analysis of NGF and BDNF expression in gWAT of vehicle- and VCD-treated mice after 3 days of CL treatment, and control mice. Two-way ANOVA revealed significant main effects of VCD treatment in BDNF expression (p = 0.0079). Significant differences between controls and VCD-treated female mice were determined by post hoc pairwise comparison with Bonferroni correction (mean ± SEM; n = 4, *p < 0.05)