Fig. 5

Sympathetic innervation levels in gWAT and iWAT of male and female mice. a, b Immunoblot analysis of tyrosine hydroxylase (TH) protein expression in gWAT and iWAT of mice treated with CL for 3 days and untreated controls. Two-way ANOVA revealed significant main effects of sex in TH protein expression (p = 0.0006). Significant differences between male and female were determined by post hoc pairwise comparison with Bonferroni correction (mean ± SEM; n = 4, *p < 0.05) (c, d). Representative images of TH and PLIN1 staining in paraffin sections of gWAT and iWAT from mice treated with CL for 3 days and untreated controls. Nuclei were counterstained with DAPI. Size bar = 20 μm