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Fig. 6 | Biology of Sex Differences

Fig. 6

From: Sexual dimorphism in the mast cell transcriptome and the pathophysiological responses to immunological and psychological stress

Fig. 6

A23187-induced degranulation and Ca2+ mobilization in female and male murine BMMCs. BMMCs were stimulated for 1 h with the Ca2+ ionophore, A23187 (1 μM). a BMMCs from females showed a 50.6% increase in β-hexosaminidase release and male BMMCs showed a 39.9% increase, a difference that was significant (P < 0.001; n = 6). b Female BMMCs released 406.1 ng/106 cells of histamine into supernatant and male BMMCs released 159.3 ng/106 cells of histamine (P < 0.001; n = 5). c Tryptic activity (ΔOD405/min/106 cells) from female BMMCs increased to 7.6 after degranulation, and tryptic activity from male BMMCs was 3.2 after degranulation (P < 0.001; n = 4). d Female BMMCs released 165.7 pg of TNF-α into supernatant and male BMMCs released 103.4 pg (P < 0.01; n = 4). All mediator release was normalized to unstimulated cells of respective sex. e Female and male BMMCs exhibited similar Ca2+ influx as measured by e Ca2+ tracings and quantified as the f change in peak fluorescence after stimulation with A23187 (1 μM). Values represent mean ± SE. **P < 0.01, ***P < 0.001 vs. males

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