Fig. 3
Female BMMCs release more preformed mediators in response to IgE-mediated degranulation. BMMCs were sensitized with anti-DNP IgE (1 μg/mL) overnight and later stimulated with DNP-HSA. a Male and female BMMCs were stimulated with 0, 15, 31, and 62 ng/mL of DNP-HSA for 1 h and female BMMCs exhibited elevated β-hexosaminidase release at all DNP concentrations (P < 0.05; n = 6). b Compared with male BMMCs, female BMMCs had an increased release of β-hexosaminidase after 30, 45, 60, and 90 min of DNP-HSA stimulus (62 ng/mL). c Female BMMCs released 141.9 ng/106 cells of histamine into supernatant and male BMMCs released 83.7 ng/106 cells of histamine after 1 h DNP stimulus (62 ng/mL) (P < 0.001; n = 5). d Tryptic activity from female BMMCs increased to 5.3 after degranulation, and tryptic activity from male BMMCs was 1.9 after degranulation with 1 h DNP stimulus (62 ng/mL) (P < 0.05; n = 6). e Female BMMCs released 107.4 pg of TNF-α into supernatant and male BMMCs released 25.5 pg after 1 h DNP stimulus (62 ng/mL) (P < 0.001; n = 5). All mediator release was normalized to unstimulated cells of respective sex. Values represent mean ± SE. # P = 0.10 *P < 0.05, **P<0.01 ***P < 0.001 vs. males