Fig. 1

Experimental design for cohorts 1, 2, and 3. a Mice in cohort 1 were sacrificed at postnatal day 21 (P21; prior to the onset of puberty). b Mice in cohorts 2 and 3 were gonadectomized (GDX) at ~15 weeks of age and implanted subcutaneously with either a testosterone (T)-filled or blank capsule. After GDX, mice in cohort 2 were left unmanipulated until they were sacrificed at 24 weeks of age. After GDX, mice in cohort 3 were exposed to 8 weeks of unpredictable chronic mild stress (UCMS), and then sacrificed (same age as cohort 2). At the time of sacrifice for all cohorts, the brains were harvested for gene expression analyses and bloods were collected for hormone assays