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Fig. 3 | Biology of Sex Differences

Fig. 3

From: Reduction of apoptosis and preservation of mitochondrial integrity under ischemia/reperfusion injury is mediated by estrogen receptor β

Fig. 3

Apoptosis evaluation. Calculation of TUNEL-positive cells within the myocardium after treatment with E2 or ERβA (a). Representative pictures of TUNEL staining and relative protein expression to measure cytochrome c level in cytosolic fraction after treatment with E2 or ERβA (b). Relative protein expression data for measurement of Bcl2 (c) and ACAA2 (d) in mitochondrial fractions as well as total caspase 9 (e) and cleavage product (f) in whole cell lysate, Data are shown mean ± SEM. Significances were calculated by ANOVA followed by post hoc Dunnet and defined as significant with *p < 0.05

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