Fig. 6
From: Characterization of osteoarthritic human knees indicates potential sex differences
Phenotypic characteristics of female and male primary osteoblasts isolated from osteoarthritic knees. First-passage osteoblasts were treated with 10−8 M 1α,25(OH)2D3 (a–c) or 10−8 M E2 (d–f). Alkaline phosphatase-specific activity was measured in whole cell lysates (a, d). Protein levels of osteocalcin (b, e) and osteoprotegerin (c, f) were measured in conditioned media. Data show treatment compared with vehicle control ratios of the responses of 6 male and 6 female patients. The dashed line represents the vehicle control (dashed line = 1). * p < 0.05 vs. control; † p < 0.05 vs. male