|
Specificity
|
Clone name
|
Fluorochrome
|
Concentration (mg/ml)
|
Dilutiona
|
Supplier
|
|---|
|
MHC2
|
2G9
|
Biotin
|
0.5
|
200×
|
BD Pharmingen ThermoFisher
|
|
Streptavidin-Pacific Orange
|
1
|
100×
|
|
F4/80
|
BM8
|
A700
|
0.5
|
75×
|
Biolegend
|
|
CD11c
|
N418
|
PE-Cy7
|
0.2
|
100×
|
Biolegend
|
|
CD11b
|
M1/70
|
APC-Cy7
|
0.2
|
200×
|
Biolegend
|
|
CD80
|
16-10A1
|
Pacific Blue
|
0.5
|
25×
|
Biolegend
|
|
CD103
|
2E7
|
PerCP-Cy5.5
|
0.2
|
25×
|
Biolegend
|
|
CD3
|
17A2
|
Pacific Blue
|
0.5
|
80×
|
Biolegend
|
|
CD8
|
53-6.7
|
A700
|
0.5
|
50×
|
Biolegend
|
|
CD25
|
PC61
|
PE-Cy7
|
0.2
|
50×
|
Biolegend
|
|
Tbet
|
eBio4B10
|
PE-Cy7
|
0.2
|
50×
|
eBioscience
|
|
Gata3
|
TWAJ
|
PerCP-Efluor710
|
0.2
|
50×
|
eBioscience
|
|
RORγt
|
B2D
|
PE
|
0.2
|
10×
|
eBioscience
|
|
FoxP3
|
FJK-16s
|
FitC
|
0.5
|
50×
|
eBioscience
|
|
NKp46
|
29A1.4
|
FitC
|
0.5
|
25×
|
Biolegend
|
|
Ly6G
|
1A8
|
Biotin
|
0.5
|
100×
|
Biolegend
|
|
Streptavidin-Pacific Orange
|
1
|
100×
|
ThermoFisher
|
|
Ly6C
|
HK1.4
|
PE
|
0.2
|
300×
|
Biolegend
|
|
MHC2
|
M5/114.15.2
|
Pacific Blue
|
0.5
|
300×
|
Biolegend
|
|
CD80
|
16-10A1
|
PerCP-Cy5.5
|
0.2
|
50×
|
Biolegend
|
-
aDilution used in a total volume of 25 μl supplemented with PBS + 10 % FCS
