Sex impacts Th1 cells, Tregs, and DCs in both intestinal and systemic immunity in a mouse strain and location-dependent manner

Table 1 Antibody specifications

Specificity	Clone name	Fluorochrome	Concentration (mg/ml)	Dilution^a	Supplier
MHC2	2G9	Biotin	0.5	200×	BD Pharmingen ThermoFisher
MHC2	2G9	Streptavidin-Pacific Orange	1	100×	BD Pharmingen ThermoFisher
F4/80	BM8	A700	0.5	75×	Biolegend
CD11c	N418	PE-Cy7	0.2	100×	Biolegend
CD11b	M1/70	APC-Cy7	0.2	200×	Biolegend
CD80	16-10A1	Pacific Blue	0.5	25×	Biolegend
CD103	2E7	PerCP-Cy5.5	0.2	25×	Biolegend
CD3	17A2	Pacific Blue	0.5	80×	Biolegend
CD8	53-6.7	A700	0.5	50×	Biolegend
CD25	PC61	PE-Cy7	0.2	50×	Biolegend
Tbet	eBio4B10	PE-Cy7	0.2	50×	eBioscience
Gata3	TWAJ	PerCP-Efluor710	0.2	50×	eBioscience
RORγt	B2D	PE	0.2	10×	eBioscience
FoxP3	FJK-16s	FitC	0.5	50×	eBioscience
NKp46	29A1.4	FitC	0.5	25×	Biolegend
Ly6G	1A8	Biotin	0.5	100×	Biolegend
Ly6G	1A8	Streptavidin-Pacific Orange	1	100×	ThermoFisher
Ly6C	HK1.4	PE	0.2	300×	Biolegend
MHC2	M5/114.15.2	Pacific Blue	0.5	300×	Biolegend
CD80	16-10A1	PerCP-Cy5.5	0.2	50×	Biolegend

ISSN: 2042-6410