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Table 1 Antibody specifications

From: Sex impacts Th1 cells, Tregs, and DCs in both intestinal and systemic immunity in a mouse strain and location-dependent manner

Specificity Clone name Fluorochrome Concentration (mg/ml) Dilutiona Supplier
MHC2 2G9 Biotin 0.5 200× BD Pharmingen ThermoFisher
Streptavidin-Pacific Orange 1 100×
F4/80 BM8 A700 0.5 75× Biolegend
CD11c N418 PE-Cy7 0.2 100× Biolegend
CD11b M1/70 APC-Cy7 0.2 200× Biolegend
CD80 16-10A1 Pacific Blue 0.5 25× Biolegend
CD103 2E7 PerCP-Cy5.5 0.2 25× Biolegend
CD3 17A2 Pacific Blue 0.5 80× Biolegend
CD8 53-6.7 A700 0.5 50× Biolegend
CD25 PC61 PE-Cy7 0.2 50× Biolegend
Tbet eBio4B10 PE-Cy7 0.2 50× eBioscience
Gata3 TWAJ PerCP-Efluor710 0.2 50× eBioscience
RORγt B2D PE 0.2 10× eBioscience
FoxP3 FJK-16s FitC 0.5 50× eBioscience
NKp46 29A1.4 FitC 0.5 25× Biolegend
Ly6G 1A8 Biotin 0.5 100× Biolegend
Streptavidin-Pacific Orange 1 100× ThermoFisher
Ly6C HK1.4 PE 0.2 300× Biolegend
MHC2 M5/114.15.2 Pacific Blue 0.5 300× Biolegend
CD80 16-10A1 PerCP-Cy5.5 0.2 50× Biolegend
  1. aDilution used in a total volume of 25 μl supplemented with PBS + 10 % FCS