Fig. 1

Gating strategy for the determination of T helper cells in the Peyer’s patches. Lymphocytes were gated on the bases of their size in the forward side scatter plot, and CD3⁺ T cells were selected. Next, CD4 and CD8 cells were selected by gating CD8 negative and positive cells, respectively. Within the CD8⁻ population, the expression of Tbet, Gata3, RORɣt, FoxP3, and CD25 were assessed. All isotype controls were set at 1 %