Fig. 2

Phenotypes of the subpopulations of alveolar macrophages based on F-actin distribution. a Punctate stain indicates scattered cytoplasmic F-actin. b F-actin is found only in the juxtanuclear region. c F-actin is diffuse in the cytoplasm. d F-actin is cortical with cytoplasmic protrusions (podosomes). The dashed lines outline the periphery of the cells. Scale bars 10 μm. e Quantification of the F-actin content in the cells. Total number of cells, n = 1627. f Cell area of the cells for each phenotype. A subset of the cells (n = 305) from e was used for area measurements. Comparisons were made by two-tailed t test with Welch’s correction (equal variances not assumed). *P < 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001