Table 8 Two-way ANOVA for univariate and pairwise comparisons between gender, estrous cycle stages, and exposure for JAK2 phosphorylation (Y1007+Y1008) expression
From: Sex-specific IL-6-associated signaling activation in ozone-induced lung inflammation
Univariate tests: dependent variable: JAK2 phosphorylated (Y1007+Y1008) | |||||||
|  |  |  | df | F | Sig. | Partial η2 | |
Analysis of gender and exposure | Exposure | FA | 1, 52 | 0.131 | 0.719 | 0.003 | |
Ozone | 1, 52 | 43.108 | <0.0005 | 0.453 | |||
Sex | F | 1, 52 | 80.967 | <0.0005 | 0.609 | ||
M | 1, 52 | 4.290 | 0.043 | 0.076 | |||
Analysis of female estrous cycle stages and exposure | Estrous cycle stage | Non-proestrus | 1, 24 | 253.484 | <0.0005 | 0.914 | |
Proestrus | 1, 24 | 5.101 | 0.033 | 0.175 | |||
Exposure | FA | 1, 24 | 0.764 | 0.391 | 0.031 | ||
Ozone | 1, 24 | 163.543 | <0.0005 | 0.872 | |||
Pairwise comparisons: dependent variable: JAK2 phosphorylated (Y1007+Y1008) | |||||||
|  |  |  | (I) Sex | (J) Sex | Mean difference (I–J) | 95 % CI for difference | |
Lower bound | Upper bound | ||||||
Analysis of gender and exposure | Exposure | FA | M | F | 0.062 | 0.281 | 0.405 |
Ozone | F | M | 1.122 | 0.779 | 1.465 | ||
Sex | F | Ozone | FA | 1.538 | 1.195 | 1.881 | |
M | Ozone | FA | 0.354 | 0.011 | 0.697 | ||
Analysis of female estrous cycle stages and exposure | Estrous cycle stage | Non-proestrus | Ozone | FA | 1.965 | 1.710 | 2.220 |
Proestrus | Ozone | FA | 0.279 | 0.024 | 0.534 | ||
Exposure | FA | Proestrus | Non-proestrus | 0.108 | 0.147 | 0.363 | |
Ozone | Non-proestrus | Proestrus | 1.578 | 1.324 | 1.833 | ||