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Table 8 Two-way ANOVA for univariate and pairwise comparisons between gender, estrous cycle stages, and exposure for JAK2 phosphorylation (Y1007+Y1008) expression

From: Sex-specific IL-6-associated signaling activation in ozone-induced lung inflammation

Univariate tests: dependent variable: JAK2 phosphorylated (Y1007+Y1008)
    df F Sig. Partial η2
Analysis of gender and exposure Exposure FA 1, 52 0.131 0.719 0.003
Ozone 1, 52 43.108 <0.0005 0.453
Sex F 1, 52 80.967 <0.0005 0.609
M 1, 52 4.290 0.043 0.076
Analysis of female estrous cycle stages and exposure Estrous cycle stage Non-proestrus 1, 24 253.484 <0.0005 0.914
Proestrus 1, 24 5.101 0.033 0.175
Exposure FA 1, 24 0.764 0.391 0.031
Ozone 1, 24 163.543 <0.0005 0.872
Pairwise comparisons: dependent variable: JAK2 phosphorylated (Y1007+Y1008)
    (I) Sex (J) Sex Mean difference (I–J) 95 % CI for difference
Lower bound Upper bound
Analysis of gender and exposure Exposure FA M F 0.062 0.281 0.405
Ozone F M 1.122 0.779 1.465
Sex F Ozone FA 1.538 1.195 1.881
M Ozone FA 0.354 0.011 0.697
Analysis of female estrous cycle stages and exposure Estrous cycle stage Non-proestrus Ozone FA 1.965 1.710 2.220
Proestrus Ozone FA 0.279 0.024 0.534
Exposure FA Proestrus Non-proestrus 0.108 0.147 0.363
Ozone Non-proestrus Proestrus 1.578 1.324 1.833