Table 1 Two-way ANOVA for univariate and pairwise comparisons between gender, estrous cycle stages, and exposure for IL6 expression
From: Sex-specific IL-6-associated signaling activation in ozone-induced lung inflammation
Univariate tests: dependent variable: IL6 | |||||||
|  |  |  | df | F | Sig. | Partial η2 | |
Analysis of gender and exposure | Exposure | FA | 1, 52 | 0.751 | 0.390 | 0.014 | |
Ozone | 1, 52 | 20.113 | <0.0005 | 0.279 | |||
Sex | F | 1, 52 | 438.088 | <0.0005 | 0.894 | ||
M | 1, 52 | 299.716 | <0.0005 | 0.852 | |||
Analysis of female estrous cycle stages and exposure | Estrous cycle stage | Non-proestrus | 1, 24 | 55.368 | <0.0005 | 0.698 | |
Proestrus | 1, 24 | 108.679 | <0.0005 | 0.819 | |||
Exposure | FA | 1, 24 | 5.228 | 0.031 | 0.179 | ||
Ozone | 1, 24 | 27.777 | <0.0005 | 0.536 | |||
Pairwise comparisons: dependent variable: IL6 | |||||||
|  |  |  | (I) Sex | (J) Sex | Mean difference (I–J) | 95 % CI for difference | |
Lower bound | Upper bound | ||||||
Analysis of gender and exposure | Exposure | FA | F | M | 0.109 | 0.144 | 0.363 |
Ozone | F | M | 0.566 | 0.313 | 0.819 | ||
Sex | F | Ozone | FA | 2.641 | 2.388 | 2.895 | |
M | Ozone | FA | 2.185 | 1.932 | 2.438 | ||
Analysis of female estrous cycle stages and exposure | Estrous cycle stage | Non-proestrus | Ozone | FA | 1.758 | 1.270 | 2.245 |
Proestrus | Ozone | FA | 2.462 | 1.975 | 2.950 | ||
Exposure | FA | Proestrus | Non-proestrus | .540 | 0.053 | 1.028 | |
Ozone | Proestrus | Non-proestrus | 1.245 | 0.757 | 1.732 | ||