Fig. 4

Expression and function of Sry copies. a Analysis of Sry transcripts (as reads per million sequence reads that contain identifying SNP) from the Rat BodyMap datasets for multiple tissues of males and a control female uterus tissue. Colors correspond to the Sry identifying SNP; Sry1 = blue, Sry2 = red, Sry3’s = green, Sry4’s = cyan, nonHMGSry = magenta. b Analysis of publically available rat RNAseq datasets (excluding the Rat BodyMap datasets) for the SNVs of Sry2 (x-axis) vs. the other non-Sry2 copies (y-axis). Tissues with the highest expression of non-Sry2 copies are labeled in red. c Differences in transcriptional regulation by the SRY2 protein. Alanine mutations to nuclear localization site of Sry1 (Sry1 20-22A) results in a loss of promoter regulation (red). Mutating amino acid 21 from His (found in Sry2) to an Arg (found in Sry1 and Sry3) in the SRY2 protein results in a significant elevation of promoter activity (blue) relative to Sry2. d, e Nuclear localization of Sry constructs shown as Western blot for multiple Sry constructs (d) or quantification of western blot for several constructs (e). For panel d, Lane 1 = Sry1(HMGbox), 2 = Sry1(delPolyQ), 3 = Sry2, 4 = Sry2(−QR), 5 = Sry1, 6 = Sry3, 7 = Sry1(20-22AAA), 8 = Sry1(78-79AA), 9 = Sry1(NoNLS), 10 = Sry1(R21H), 11 = Sry2(H21R), 12 = negative control (empty vector)