Fig. 6

Masculinization of the spinal GRP system in female neonates exposed to androgens. Representative photomicrographs of female neonates treated with 5α-dihydrotestosterone (DHT). Neonatal administration of DHT increased the number and intensity of GRP-immunoreactive neurons (left panels). Immunohistochemical analysis of GRP (green) and nNOS (magenta) showing significantly increased intensity of GRP-immunoreactive fibers in the SPN induced by neonatal administration of DHT. Arrowheads indicate possible GRP-positive cell bodies. Scale bars 100 μm, left panel; 50 μm, right panel