Fig. 2From: Perinatal testosterone exposure is critical for the development of the male-specific sexually dimorphic gastrin-releasing peptide system in the lumbosacral spinal cord that mediates erection and ejaculationFlutamide administered to neonatal male rats demasculinizes the spinal GRP system. Flutamide decreased the number and intensity of GRP-immunoreactive neurons (left panels). Immunohistochemical analysis of the expression of GRP (green) and neuronal nitric oxide synthase (magenta) show a decrease in the intensity of GRP-immunoreactive fibers in the SPN. Arrowheads indicate possible GRP-positive cell bodies. Scale bars 100 μm, left panel; 50 μm, right panel Back to article page