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Fig. 4 | Biology of Sex Differences

Fig. 4

From: Sex-specific regulation of chemokine Cxcl5/6 controls neutrophil recruitment and tissue injury in acute inflammatory states

Fig. 4

Cxcl5 induces neutrophil stimulation and mobilization pathways. ad Male rats were administered 3 μg/kg Cxcl5 (ip, n = 5 rats) or sterile PBS (n = 3 rats) for 2 h. a Circulating RP1+ neutrophils and CD68+ monocytes. b Expression of β1 and β2 integrins on bone marrow RP1+ neutrophils. c Mesenteric necrosis, measured by nitroblue tetrazolium and d lung MPO levels. (ef, hi) Bone marrow cells (5 × 105) were isolated from male and female rats (n = 4 rats/group) and treated with Cxcl5 (10 ng/ml) or Cxcl1 (10 ng/ml) for 2 h. e Bone marrow cell GCSF mRNA expression. f Expression of neutrophil β2 integrin, measured by cytometry. g Isolated male and female bone marrow cells (105) were stimulated with Cxcl5 (100 ng/ml, 1 h) prior to incubation in 96-well plates for 40 min. Adhesion of cells to wells coated with BSA (2 %) or ICAM1 (1 μg/well), quantified using calcein-AM (n = 3 rats/group). hi Bone marrow cell mRNA expression of h Cxcr2, i Cxcl5. Levels of mRNA are normalized to 18S and calculated as fold expression relative to mean value of male control group. Data are presented as mean ± sem. *P < 0.05, **P < 0.01, ***P < 0.001, ns P > 0.05 by one-way ANOVA

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