Fig. 2
Increased bone marrow neutrophil stimulation in males. Male and female rats were subjected to 30-min mesenteric ischemia followed by up to 2-h reperfusion. a Expression of mesenteric tissue and bone marrow cell GCSF mRNA, normalized to levels of 18S and calculated as fold expression relative to mean value of male sham group. b Bone marrow neutrophil shape change measured by cytometry as the proportion of RP1+ cells with high forward scatter (FSChi), at 2-h reperfusion. c Bone marrow and blood neutrophil β2 integrin expression at 2-h reperfusion, measured by cytometry and expressed as relative fluorescence intensity (RFI) compared to isotype control antibody. d Neutrophil accumulation in the lungs, assessed by tissue MPO activity. Sham n = 5 rats/group, I/R n = 8 rats/group. Data are presented as mean ± sem. *P < 0.05, **P < 0.01, ***P < 0.001, and ns P > 0.05 by one-way ANOVA