Table 1 Fluorophore conjugated proteins/antibodies used to characterize blood-borne microvesicles by flow cytometry
From: Age- and sex-specific differences in blood-borne microvesicles from apparently healthy humans
Parameters | Protein/antibodies used for characterization | |
Procoagulant (or) thrombogenic microvesicles | ||
Phosphatidylserine positive | Recombinant annexin-V | |
Tissue factor positive | Tissue factor | |
P-selectin positive | P-selectin | |
Anticoagulant microvesicles | ||
Tissue factor pathway inhibitor (TFPI) positive | TFPI | |
Cellular adhesion molecules positive microvesicles | ||
Inter cellular adhesion molecule-1 (ICAM-1) positive | ICAM-1 | |
Vascular cell adhesion molecule-1(VCAM-1) positive | VCAM-1 | |
Cellular origin of microvesicles | Marker 1: protein/antibodies | Marker 2: protein/antibodies |
Erythrocyte-derived | CD235a | CD236 |
Leukocyte-derived | CD45 | CD11c |
Monocyte-derived | CD14 | CD86/CD68 |
T-cell-derived | CD3 | CD2/CD69 |
B-cell-derived | CD19 | CD70 |
Platelet-derived | CD42a | CD41 |
Endothelium-derived | CD62E | CD146/CD144 |
Adipocyte-derived | Fatty-acid-binding protein 4 (FABP4) | Pre-adipocyte factor-1 (Pref-1) |
Stem/progenitor cell-derived | CD117 | CD34 |
Senescent cell-derived | p16-set | Alpha-L-fucosidase |