Figure 5

Podocyte markers displaying high intra-individual correlation. The upper left is the correlation of two different fluorophores conjugated to annexin-V and binding to surface phosphatidylserine on EVs from the same urine sample. The upper middle is the correlation of two distinct antibodies for the same cell-specific antigens (e.g., podocytes) binding to urinary EVs from the same urine sample. The upper right shows that annexin-V-positive vesicles did not correlate with urine osmolality. There were also no differences in the concentration of annexin-V- (lower left) and exosome marker (CD63, lower right)-positive EVs between two different 24-h urine collections obtained within 3 months from the same individuals.