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Figure 3 | Biology of Sex Differences

Figure 3

From: Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study

Figure 3

Representative typical scatter plots (A–C) and fluorescence dot (quadrants) plots (D–F) from FACSCanto™ flow cytometry, respectively. (A) Control gates of buffer with fluorophore-conjugated antibodies and calibration beads. (B) Gates derived from diluted urine plus appropriate fluorophore-conjugated antibodies and calibration beads from one individual. (C) Gates derived from diluted urine plus appropriate fluorophore-conjugated antibodies and calibration beads from another individual. (D) Fluorescent dot plot (quadrants derived from microvesicle gate of Figure 3B) showing fluorophore spectra separate of annexin-V and P-selectin (activated platelet specific protein, irrelevant antibody control used for urine EV analysis). A similar pattern was observed with isotype control (fluorophore conjugated IgG) antibodies in diluted urine samples (data not shown). (E) Fluorescent dot plot (quadrants derived from microvesicle gate of Figure 3B) showing fluorophore spectra separate of annexin-V and CD9 antibody. (F) Fluorescent dot plot (quadrants derived from microvesicle gate of Figure 3B) showing fluorophore spectra separate of annexin-V and aquaporin-2 (a specific marker for collecting duct cell-derived EVs) fluorophore-positive (+) and fluorophore-negative (−) EVs. Similar fluorescent dot plots were obtained from diluted urine stained with specific antibodies for cells of the urinary tract (Figure 4, data not shown).

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