NOS3 mRNA expression and NOS3 protein expression in FHUVECs and MHUVECs. (A) Relative NOS3 mRNA expression. The values are expressed as the means ± SEM (*P = 0.036) of four independent experiments for each sex. (B) Representative Western blot and densitometric analysis of NOS3 expression. The values are expressed as medians + MAD (*P < 0.05) of 17 independent experiments for each sex normalised to actin levels.