Mean arterial pressure response to angiotensin II infusion. Mean arterial pressure (MAP) during baseline and 14-day infusion of vehicle (0.9% saline, n = 4–8 per group; open symbols) in (A) adult, (B) aged, and (C) aged-OVX or AngII (600 ng/kg/min, n = 6–8 per group; closed symbols) in (D) adult, (E) aged, and (F) aged-OVX WT (black symbols) and AT2R-KO (grey symbols) female mice. (G) The change in MAP from baseline at day 14 of AngII infusion in WT (black symbols) and AT2R-KO (grey symbols) female mice. Data are presented as 24 h mean ± SEM. Data in (A–F) were analyzed using repeated measures ANOVA. The change in MAP on day 14 of AngII infusion was analyzed using a one-way ANOVA followed by post hoc t-tests with Holm-Sidak correction to reduce the risk of type 1 error associated with multiple comparisons. *P < 0.05; **P < 0.01.