Expression and knockdown of dsx in the A. aegypti pupal brain. (A) Detection of dsx expression in developing wild-type female (top) and male (bottom) pupal brain hemisegments with riboprobes corresponding to exon 2 (dsx common), DsxM (dsx male), and DsxF (dsx female). At right in (A), a brain processed with a sense control probe lacks staining. Knockdown of both splice variants was detected through use of the dsx common (B), dsxF (C, top), and dsxM (C, bottom) probes in female (top in B, C) and male (bottom in B, C) pupal brain hemisegments following injection of dsx KD A or KD B siRNAs. Control siRNA-injected animals (control in B, C) resemble wild-type animals. Brain hemisegments are oriented dorsal upward in each panel.