Sex-specific expression patterns of DETs in sectioned A. aegypti pupal brains. Differential expression of cdk4/6 (A, E) , geko (B, F) , synj (C, G), and p53 (D, H) was detected in 12 μ sections through 24 hr female (A-D) and male (E-H) pupal brains. Hemisegments oriented dorsal upward are shown. Hybridizations with a sense control probe detected no signal in comparable brain sections (not shown). cdk4/6 is commonly expressed in the optic lobe (blue arrowheads in A, E), but males have an additional cdk4/6 expression domain in the ventral suboesophageal ganglion (red arrowhead in E). geko, which is commonly expressed in the female and male optic lobe (blue arrowheads in B, F), is expressed in additional large cell bodies near the female midbrain and antennal lobe (red arrowheads in B). synj expression is detected in the optic lobe (blue arrowheads in C, G) and in a subset of midbrain neurons (red arrowheads in C, G). The red arrowhead in G marks sex-specific synj optic lobe expression in males, and midbrain synj levels are generally higher in males (compare expression adjacent to red arrowheads in C, G). p53 is expressed in the optic lobe and suboesophageal ganglion of females (blue and red arrowheads, respectively in D). p53 is also expressed in the male optic lobe (blue arrowheads in H), but not in the male subesophageal ganglion (H). Male-specific p53 expression is detected in neurons adjacent to the antennal lobe (red arrowheads in H). These data are consistent with the results presented in Figure 7. al antennal lobe, la lamina, me medulla, SuEG, supraesophageal ganglion.