Sex-specific expression of DETs in the A. aegypti pupal brain. Differential expression of the indicated genes was confirmed in female vs. male 24 h pupal brains through whole mount in situ hybridization experiments. In agreement with the whole head microarray experimental data, these experiments demonstrated that takeout, ci, cdk4/6, obp10, arrow, and caspase7 (top row, A-F respectively) are upregulated in the female 24 h pupal brain, while synj, geko, p53, and obp13 are upregulated in the male 24 h pupal brain (G-J, respectively). A brain processed with a sense control probe lacks staining (K). For a number of the genes assayed (takeout, ci, obp10, arrow, caspase 7, and obp13), dimorphic gene expression localized to the optic lobe, which is marked by an asterisk in (A) and located at a similar position in other panels. Brain hemisegments are oriented dorsal upward in each panel.