Sex-specific expression patterns of dsx in the A. aegypti pupal brain. The precise locations of dsx expression in the 24 h female (A, C, E) and male (B, D, F) pupal brain were also mapped through co-labeling experiments with dsx common probe (left in panels A-F; red in overlays at right) and anti-HRP staining (center panels of A-F; green in overlays at right), which marks various neuropil regions in the developing A. aegypti brain. Confocal imaging was performed following staining of whole mount brains. Five merged Z-stacks of confocal sections (totaling 5 μ) through the optic lobe (A, B), antennal lobe (C,D), and mushroom body (E, F) regions of brain hemisegments oriented dorsal upward are shown. dsx expression is detected in the 24 h female pupal optic lobe (A), throughout the antennal lobe (C), and in the mushroom body (E). dsx expression is detected in the 24 h male pupal optic lobe (B) and a group of ventral-most cells in the antennal lobe (D), but not in the mushroom body (F). These data are consistent with the results presented in Figure 9.