| Two-way RM ANOVA (treatment × estrous) | ||||||
---|---|---|---|---|---|---|---|
BL | Day 7 | Day 13 | Day 21 | Day 29 | Day 35 | Day 42 | |
Clinical scores, Figure 5A |  |  |  |  |  |  |  |
 Treatment | F1,28 = 0.2, P = 0.6 | F1, 27 = 2.0, P = 0.16 | F1,30 = 1.09, P = 0.3 | F1,30 = 10.3, P < 0.05 | F1,31 = 8.5, P < 0.01 | F1,31 = 0.5, P = 0.4 | F1,31 = 2.2, P = 0.1 |
 Estrous | F1,28 = 0.2, P = 0.6 | F1, 27 = 0.5, P = 0.4 | F1,30 = 0.2, P = 0.5 | F1,30 = 0.6, P = 0.4 | F1,31 = 1.1, P = 0.2 | F1,31 = 3.0, P = 0.09 | F1,31 = 2.8, P = 0.053 |
 Treatment × estrous | F1,28 = 0.2, P = 0.6 | F1, 27 = 0.001, P = 0.9 | F1,30 = 1.09, P = 0.3 | F1,30 = 0.6, P = 0.4 | F1,31 = 0.2, P = 0.9 | F1,31 = 3.7, P = 0.6 | F1,31 = 3.7, P < 0.05 |
Grip strength, Figure 5C |  |  |  |  |  |  |  |
 Treatment | F1,28 = 0.3, P = 0.5 | F1. 27 = 2.7, P = 0.1 | F1,30 = 0.09, P = 0.7 | F1,30 = 11.0, P < 0.05 | F1,31 = 19.8, P < 0.001 | F1,31 = 5.8, P < 0.05 | F1,31 = 6.4, P < 0.05 |
 Estrous | F1,28 = 0.04, P = 0.8 | F1, 27 = 0.7, P = 0.3 | F1,30 = 0.6, P = 0.4 | F1,30 = 0.002, P = 0.9 | F1,31 = 0.05, P = 0.8 | F1,31 = 0.1, P = 0.7 | F1,31 = 0.1, P = 0.7 |
 Treatment × estrous | F1,28 = 0.3, P = 0.5 | F1, 27 = 0.06, P = 0.8 | F1,30 = 2.1, P = 0.15 | F1,30 = 0.5, P = 0.4 | F1,31 = 0.02, P = 0.8 | F1,31 = 0.009, P = 0.9 | F1,31 = 0.02, P = 0.8 |
Mechanical thresholds, Figure 5E |  |  |  |  |  |  |  |
 Treatment | F1,28 = 0.08, P = 0.7 | F1, 27 = 4.2, P < 0.05 | F1,30 = 0.7, P = 0.3 | F1,30 = 7.4, P < 0.05 | F1,31 = 25.9, P < 0.001 | F1,31 = 2.0, P = 0.16 | F1,31 = 3.5, P = 0.07 |
 Estrous | F1,28 = 0.9, P = 0.3 | F1, 27 = 0.4, P = 0.4 | F1,30 = 2.3, P = 0.13 | F1,30 = 0.09, P = 0.7 | F1,31 = 5.2, P < 0.05 | F1,31 = 3.1, P = 0.08 | F1,31 = 0.2, P = 0.6 |
 Treatment × estrous | F1,28 = 0.001, P = 0.9 | F1, 27 = 0.09, P = 0.7 | F1,30 = 0.1, P = 0.7 | F1,30 = 1.5, P = 0.2 | F1,31 = 8.7, P < 0.01 | F1,31 = 2.1, P = 0.14 | F1,31 = 2.6, P = 0.11 |
Acetone withdrawal, Figure 5G |  |  |  |  |  |  |  |
 Treatment | F1,28 = 0.1, P = 0.7 | F1,27 = 1.9, P =0.17 | F1,30 = 4.2, P < 0.05 | F1,30 = 4.2, P < 0.05 | F1,31 = 5.2, P < 0.05 | F1,31 = 1.0, P = 0.3 | F1,31 = 7.2, P < 0.05 |
 Estrous | F1,28 = 0.01, P = 0.9 | F1,27 = 0.000, P = 0.9 | F1,30 = 0.01, P = 0.9 | F1,30 = 0.02, P = 0.6 | F1,31 = 0.001, P = 0.9 | F1,31 = 0.8, P = 0.7 | F1,31 = 0.09, P = 0.7 |
 Treatment × estrous | F1,28 = 0.9, P = 0.3 | F1,27 = 0.06, P = 0.7 | F1,30 = 0.001, P = 0.9 | F1,30 = 1.2, P = 0.2 | F1,31 = 0.2, P = 0.6 | F1,31 = 3.7, P = 0.06 | F1,31 = 6.3, P < 0.05 |
AUC | Clinical scores: | Â | Grip strength: | Â | Mechanical thresholds: | Â | Acetone withdrawal: |
one-way ANOVA: | Â | one-way ANOVA: | Â | one-way ANOVA: | Â | one-way ANOVA: | |
F3,44 = 8.8, P < 0.001 |  | F3,44 = 5.3, P < 0.01 |  | F3,44 = 5.8, P < 0.01 |  | F3,44 = 2.8, P < 0.05 | |
 | (Figure 5B) |  | (Figure 5D) |  | (Figure 5F) |  | (Figure 5H) |