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Table 3 Statistics for experiment 2 to determine the effects of estrous phase (proestrus vs. non-proestrus phases)

From: Sex differences in a mouse model of multiple sclerosis: neuropathic pain behavior in females but not males and protection from neurological deficits during proestrus

 

Two-way RM ANOVA (treatment × estrous)

BL

Day 7

Day 13

Day 21

Day 29

Day 35

Day 42

Clinical scores, Figure 5A

       

 Treatment

F1,28 = 0.2, P = 0.6

F1, 27 = 2.0, P = 0.16

F1,30 = 1.09, P = 0.3

F1,30 = 10.3, P < 0.05

F1,31 = 8.5, P < 0.01

F1,31 = 0.5, P = 0.4

F1,31 = 2.2, P = 0.1

 Estrous

F1,28 = 0.2, P = 0.6

F1, 27 = 0.5, P = 0.4

F1,30 = 0.2, P = 0.5

F1,30 = 0.6, P = 0.4

F1,31 = 1.1, P = 0.2

F1,31 = 3.0, P = 0.09

F1,31 = 2.8, P = 0.053

 Treatment × estrous

F1,28 = 0.2, P = 0.6

F1, 27 = 0.001, P = 0.9

F1,30 = 1.09, P = 0.3

F1,30 = 0.6, P = 0.4

F1,31 = 0.2, P = 0.9

F1,31 = 3.7, P = 0.6

F1,31 = 3.7, P < 0.05

Grip strength, Figure 5C

       

 Treatment

F1,28 = 0.3, P = 0.5

F1. 27 = 2.7, P = 0.1

F1,30 = 0.09, P = 0.7

F1,30 = 11.0, P < 0.05

F1,31 = 19.8, P < 0.001

F1,31 = 5.8, P < 0.05

F1,31 = 6.4, P < 0.05

 Estrous

F1,28 = 0.04, P = 0.8

F1, 27 = 0.7, P = 0.3

F1,30 = 0.6, P = 0.4

F1,30 = 0.002, P = 0.9

F1,31 = 0.05, P = 0.8

F1,31 = 0.1, P = 0.7

F1,31 = 0.1, P = 0.7

 Treatment × estrous

F1,28 = 0.3, P = 0.5

F1, 27 = 0.06, P = 0.8

F1,30 = 2.1, P = 0.15

F1,30 = 0.5, P = 0.4

F1,31 = 0.02, P = 0.8

F1,31 = 0.009, P = 0.9

F1,31 = 0.02, P = 0.8

Mechanical thresholds, Figure 5E

       

 Treatment

F1,28 = 0.08, P = 0.7

F1, 27 = 4.2, P < 0.05

F1,30 = 0.7, P = 0.3

F1,30 = 7.4, P < 0.05

F1,31 = 25.9, P < 0.001

F1,31 = 2.0, P = 0.16

F1,31 = 3.5, P = 0.07

 Estrous

F1,28 = 0.9, P = 0.3

F1, 27 = 0.4, P = 0.4

F1,30 = 2.3, P = 0.13

F1,30 = 0.09, P = 0.7

F1,31 = 5.2, P < 0.05

F1,31 = 3.1, P = 0.08

F1,31 = 0.2, P = 0.6

 Treatment × estrous

F1,28 = 0.001, P = 0.9

F1, 27 = 0.09, P = 0.7

F1,30 = 0.1, P = 0.7

F1,30 = 1.5, P = 0.2

F1,31 = 8.7, P < 0.01

F1,31 = 2.1, P = 0.14

F1,31 = 2.6, P = 0.11

Acetone withdrawal, Figure 5G

       

 Treatment

F1,28 = 0.1, P = 0.7

F1,27 = 1.9, P =0.17

F1,30 = 4.2, P < 0.05

F1,30 = 4.2, P < 0.05

F1,31 = 5.2, P < 0.05

F1,31 = 1.0, P = 0.3

F1,31 = 7.2, P < 0.05

 Estrous

F1,28 = 0.01, P = 0.9

F1,27 = 0.000, P = 0.9

F1,30 = 0.01, P = 0.9

F1,30 = 0.02, P = 0.6

F1,31 = 0.001, P = 0.9

F1,31 = 0.8, P = 0.7

F1,31 = 0.09, P = 0.7

 Treatment × estrous

F1,28 = 0.9, P = 0.3

F1,27 = 0.06, P = 0.7

F1,30 = 0.001, P = 0.9

F1,30 = 1.2, P = 0.2

F1,31 = 0.2, P = 0.6

F1,31 = 3.7, P = 0.06

F1,31 = 6.3, P < 0.05

AUC

Clinical scores:

 

Grip strength:

 

Mechanical thresholds:

 

Acetone withdrawal:

one-way ANOVA:

 

one-way ANOVA:

 

one-way ANOVA:

 

one-way ANOVA:

F3,44 = 8.8, P < 0.001

 

F3,44 = 5.3, P < 0.01

 

F3,44 = 5.8, P < 0.01

 

F3,44 = 2.8, P < 0.05

 

(Figure 5B)

 

(Figure 5D)

 

(Figure 5F)

 

(Figure 5H)

  1. Treatment conditions: CFA, n = 17; CFA + MOG35-55, n = 18. Estrous phase: proestrus (n = 4–18/day) or non-proestrus phases (n = 17–30/day). AUC area under the curve.