Proestrus produces protective effects in the EAE model at late time points. Neurological motor deficits (A), grip strength (C), mechanical withdrawal thresholds (E), and responses to acetone application (G) in female mice analyzed across phases of the estrous cycle. Area under the curve (AUC) analyses examining neurological motor deficits (B), grip strength (D), and sensitivity to mechanical (F) and cold (H) stimulation in females analyzed across the phases of the estrous cycle. Non-proestrus includes the following phases: diestrus, metestrus, and estrus. BL baseline. Values represent mean ± SEM. ***P < 0.001, **P < 0.01, *P < 0.05 main effect of treatment as indicated (two-way ANOVA comparing treatment by estrous). ★★★P < 0.001, ★★P < 0.01, ★P < 0.05 comparison as indicated (one-way ANOVA and Bonferroni correction). N = 3–16/group, except n = 2 in groups CFA proestrus and MOG35-55 proestrus on day 13.