EAE induces mechanical and cold hypersensitivity in female, but not male C57BL/6 mice. Administration of MOG35-55 increased sensitivity to mechanical and cold stimulation in females (A and C, respectively), but not their male counterparts (B and D, respectively) relative to CFA sex- and age-matched controls. MOG35-55 did not alter paw withdrawal latencies in response to radiant heat in either female (E) or male (F) mice as compared to CFA age- and sex-matched controls. Area under the curve (AUC) analyses on days 7–21 examining sensitivity to mechanical (G) and cold (H) and days 10–16 examining thermal stimulation (I) in females (F) and males (M). MOG35-55 injections began on day 0. BL baseline. Values represent mean ± SEM. ★★★P < 0.001, ★★P < 0.01, and ★P < 0.05 compared to sex- and age-matched CFA control (one-way ANOVA) or comparison as indicated in AUC figures (one-way ANOVA and Bonferroni Correction). N = 10–13/group.