Estrogen increased the expression of miR-223 and miR-451 expression in splenocytes from NZB/W
mice. The graphs show increased miR-223 and miR-451 expression in splenocytes from 10-wk-old (A) and 32-wk-old (B) estrogen-treated orchidectomized NZB/WF1 mice when compared to either age-matched placebo control or intact control mice. The graph shows the means ± SEMS (n = 2 for 32-wk-old, estrogen-treated mice group, and n = 4 each for the other groups). Student t test was performed (estrogen vs placebo or intact). *p < 0.05 and *** p <0.001.