Comparable lupus-associated miRNA levels in splenocytes from male and female NZB/W
mice before lupus onset. (A) Real-time RT-PCR analysis of select lupus-associated miRNAs. The graph represents the means ± SEMs (n = 4 each). Student t test was performed (male vs female). *p < 0.05. (B) ELISA assay of serum anti-dsDNA autoantibodies. The serum samples from three non-autoimmune C57/BL6 mice were included as negative control. The mean serum anti-DNA autoantibody value in each group was indicated by black line.